Cell and Protein Characterization Core

Cell and Protein Characterization Core focuses on resolving the cellular basis of cancer. Each individual tumour can be characterized for the physical appearance of the cells and specific proteins that they contain. This provides information about what kind of cancer it is. DNA changes can lead to protein changes and this service can give clues as to which type of treatments are best for the patient.

Lead Investigators
  • Dr. Bulent Mutus, Chemistry & Biochemistry, University of Windsor
  • Dr. Otis Vacratsis, Chemistry & Biochemistry, University of Windsor
  • Dr. Lisa A. Porter, Biological Sciences, University of Windsor
  • Dr. James Gauld, Chemistry & Biochemistry, University of Windsor
  • Dr. Andrew Swan, Biological Sciences, University of Windsor
Equipment Available
  • Flow cytometry core (see porterlab.ca) – determines the exact composition of cells in a tumour – can sort out stem cells and healthy vs diseased cells
  • Leica microscope and 3 laser confocal scope – can look at individual cells and monitor behaviour or changes in appearance
  • Alpha innotech imager – determines changes in proteins within a specific tissue sample
  • Cyclone phosphorimager – measures radioactivity in a sample
  • High throughput mass spectrometry – The main objective of proteomics is to relate the expression levels of proteins to cellular function. Proteomics is complementary to next generation genomic sequencing and can be used for discovering novel disease biomarkers, analyzing biomarkers for diagnostic purposes, and evaluating the cellular effects of drug treatments.
Core Needs
  • Next generation proteomics system – mass spectrometer with automated peripheral equipment. Next generation proteomic systems consists of high resolution mass spectrometers coupled with peripheral equipment for sample preparation and data analysis.
  • In vivo animal imaging – TEM/SEM and cryoTEM
  • Confocal microscope with UV imaging capabilities
  • Plate reader (luminescence, fluorescence, colorimetric)
  • Computer systems to hold big data
  • Odyssey-Clx by LiCOR
  • MSD Multiarray platform
  • Slide scanner
  • Tissue processor for paraffin embedding; microtome